不同训练方式建立大鼠空间记忆后海马结构中BDNF表达的变化

第44卷第1期第16页2015年2月 华中科技大学学报(医学版)Acta M ed U niv Sci T echnol Huazhong Vol .44 No .1 P .16Feb . 2015

*教育部中国博士后科学基金资助项目(No 。2013M 540760)

于 玮,女,1978年生,硕士研究生,讲师,E ‐mail :marine _w ei @163.com

△通讯作者,Corresponding author ,E ‐mail :zhangli 1998@mail .xjtu .edu .cn 不同训练方式建立大鼠空间记忆后海马

结构中B D N F 表达的变化*

于 玮1, 谢 雯2, 罗 禹3, 张 莉2,3

△1

西安医学院生理学教研室,西安 7100212

西安交通大学医学院生理学与病理生理学系,西安 7100613西安交通大学环境与疾病相关基因教育部重点实验室,西安 710061

摘要:目的 研究短期强化训练能否建立可靠的空间长时记忆;用不同训练方式建立空间记忆后,大鼠海马结构中

脑源性神经营养因子(BDNF )的表达是否会发生变化。方法 利用Morris 水迷宫建立大鼠空间短期记忆、长期记忆和短期强化训练形成的长期记忆3种模型,用激光共聚焦显微镜观察正常(NC )组、短期记忆(ST )组、长期记忆组(LT )和短期强化训练形成的长期记忆(SRT )组大鼠海马各亚区的BDNF 的分布情况。结果 Morris 水迷宫定位航行实验中LT 组和SRT 组间寻找站台平均潜伏期和策略差异均无统计学意义;记忆检测发现,LT 组大鼠除了在站台所在象限的停留时间明显长于SRT 组,差异有统计学意义(P <0。05)外,两组大鼠寻找站台潜伏期和策略以及穿越站台次数差异均无统计学意义。LT 组与NC 组及ST 组比较,海马齿状回和CA 1区的BDNF 免疫荧光反应明显增强,差异有统计学意

义(均P <0.05);SRT 组与NC 组以及ST 组比较,海马齿状回和CA 1区的BDNF 免疫荧光反应明显增强,差异有统计学意义(P <0.05或P <0.01)。LT 组与SRT 组之间,NC 组与ST 组之间,海马各区的BDNF 表达差异均无统计学意义。结论 短期强化训练可建立与长期训练基本相同的长期记忆。BDNF 与空间长期记忆的形成密切相关,其可能不参与空间短期记忆的形成或调节。

关键词:脑源性神经营养因子; 海马; 学习记忆

中图分类号:G 804.7,R 49 DOI :10.3870/j .issn .1672‐0741.2015.01.003Changes of BDNF Expression in Rat Hippocampus after Establishment of Spatial Memory by Different Trainings

Yu Wei 1,Xie Wen 2,Luo Yu 3et al 1De p artment o f Ph y siology ,X i ’an Medical Universit y ,X i ’an 710021,China 2

De p artment o f Ph y siology and Patho p hysiology ;3Ke y L aborator y o f Env ironment and Gene Related Diseases o f the M inistr y o f Education ,Medical School o f X i ’an Jiaoton g Universit y ,X i ’an 710061,China Abstract Objective To investigate whether short reinforcing training can establish stable spatial long ‐term memory and how the brain ‐derived neurotrophic factor (BDNF )expression in the hippocampus changes after the establishment of spatial memory with different trainings 。Methods Long ‐term memory was established by spatial short ‐term memory training (ST g roup ),spatial long ‐term memory training (LT group )or short reinforcing training (SRT group )in Morris water maze 。Laser scanning confocal microscopy was used to observe the distribution of BDNF in each area of the hippocampus in normal control g roup (NC ),ST ,LT and SRT groups 。Results Morris water maze testing showed that there was no significant difference in the mean latency and the target ‐seeking strategies between the LT and SRT groups 。The examination of the memories following dif ‐ferent trainings indicated that the mean latency and the target ‐seeking strategies as well as the times of crossing the target in LT g roup were not significantly different from those in SRT group ,except that the mean time remaining in the platform quadrant in LT group was significantly longer than that in SRT group (P <0。05)。The integral absorbance (A )value of BDNF in the dentate gy rus (DG )and CA 1area of the hippocampus was significantly increased in LT and SRT groups compared with that in NC and ST groups (P <0。05or P <0。01)。In addition ,no significant difference in the integral A value of BDNF in CA 1,CA 3and DG of the hippocampus was observed between NC and ST groups or between LT and SRT groups 。Conclusion The short reinforcing training can establish long ‐term spatial memory ,which is almost the same as that established by the long ‐term training 。BDNF is closely associated with spatial long ‐term memory formation and it may not be involved in the formation or regulation of spatial short ‐term memory 。Key words brain ‐derived neurotrophic factor ; hippocampus ; learning and memory

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